diff --git a/sys/dev/ena/ena.c b/sys/dev/ena/ena.c
index dbe5b9f785a9..faa1278c6d81 100644
--- a/sys/dev/ena/ena.c
+++ b/sys/dev/ena/ena.c
@@ -2092,6 +2092,8 @@ ena_up(struct ena_adapter *adapter)
 {
 	int rc = 0;
 
+	ENA_LOCK_ASSERT(adapter);
+
 	if (unlikely(device_is_attached(adapter->pdev) == 0)) {
 		ena_log(adapter->pdev, ERR, "device is not attached!\n");
 		return (ENXIO);
@@ -2465,6 +2467,8 @@ ena_down(struct ena_adapter *adapter)
 {
 	int rc;
 
+	ENA_LOCK_ASSERT(adapter);
+
 	if (!ENA_FLAG_ISSET(ENA_FLAG_DEV_UP, adapter))
 		return;
 
diff --git a/sys/dev/ena/ena.h b/sys/dev/ena/ena.h
index 1d06a3cb56de..f05c2d69fbe4 100644
--- a/sys/dev/ena/ena.h
+++ b/sys/dev/ena/ena.h
@@ -480,12 +480,16 @@ struct ena_adapter {
 #define	ENA_RING_MTX_LOCK(_ring)		mtx_lock(&(_ring)->ring_mtx)
 #define	ENA_RING_MTX_TRYLOCK(_ring)		mtx_trylock(&(_ring)->ring_mtx)
 #define	ENA_RING_MTX_UNLOCK(_ring)		mtx_unlock(&(_ring)->ring_mtx)
+#define ENA_RING_MTX_ASSERT(_ring)		\
+	mtx_assert(&(_ring)->ring_mtx, MA_OWNED)
 
 #define ENA_LOCK_INIT(adapter)			\
 	sx_init(&(adapter)->global_lock, "ENA global lock")
 #define ENA_LOCK_DESTROY(adapter)	sx_destroy(&(adapter)->global_lock)
 #define ENA_LOCK_LOCK(adapter)		sx_xlock(&(adapter)->global_lock)
 #define ENA_LOCK_UNLOCK(adapter)	sx_unlock(&(adapter)->global_lock)
+#define ENA_LOCK_ASSERT(adapter)		\
+	sx_assert(&(adapter)->global_lock, SA_XLOCKED)
 
 #define clamp_t(type, _x, min, max)	min_t(type, max_t(type, _x, min), max)
 #define clamp_val(val, lo, hi)		clamp_t(__typeof(val), val, lo, hi)
diff --git a/sys/dev/ena/ena_datapath.c b/sys/dev/ena/ena_datapath.c
index e6286ebfc25f..6506c808e4cb 100644
--- a/sys/dev/ena/ena_datapath.c
+++ b/sys/dev/ena/ena_datapath.c
@@ -1075,6 +1075,8 @@ ena_start_xmit(struct ena_ring *tx_ring)
 	int ena_qid;
 	int ret = 0;
 
+	ENA_RING_MTX_ASSERT(tx_ring);
+
 	if (unlikely((if_getdrvflags(adapter->ifp) & IFF_DRV_RUNNING) == 0))
 		return;