diff --git a/app/test-bbdev/test-bbdev.py b/app/test-bbdev/test-bbdev.py
index 2d1f1dfc55..291c80b0f5 100755
--- a/app/test-bbdev/test-bbdev.py
+++ b/app/test-bbdev/test-bbdev.py
@@ -1,9 +1,8 @@
-#!/usr/bin/env python
+#!/usr/bin/env python3
 
 # SPDX-License-Identifier: BSD-3-Clause
 # Copyright(c) 2017 Intel Corporation
 
-from __future__ import print_function
 import sys
 import os
 import argparse
@@ -16,10 +15,6 @@ def kill(process):
     print("ERROR: Test app timed out")
     process.kill()
 
-if sys.version_info.major < 3:
-    print("WARNING: Python 2 is deprecated for use in DPDK, and will not work in future releases.", file=sys.stderr)
-    print("Please use Python 3 instead", file=sys.stderr)
-
 dpdk_path = "../.."
 dpdk_target = "build"
 
diff --git a/app/test-cmdline/cmdline_test.py b/app/test-cmdline/cmdline_test.py
index 954428e2bf..f3377313e9 100755
--- a/app/test-cmdline/cmdline_test.py
+++ b/app/test-cmdline/cmdline_test.py
@@ -1,9 +1,8 @@
-#!/usr/bin/env python
+#!/usr/bin/env python3
 # SPDX-License-Identifier: BSD-3-Clause
 # Copyright(c) 2010-2014 Intel Corporation
 
 # Script that runs cmdline_test app and feeds keystrokes into it.
-from __future__ import print_function
 import cmdline_test_data
 import os
 import pexpect
@@ -19,10 +18,6 @@ def runTest(child, test):
         return 0
     child.expect(test["Result"], 1)
 
-if sys.version_info.major < 3:
-    print("WARNING: Python 2 is deprecated for use in DPDK, and will not work in future releases.", file=sys.stderr)
-    print("Please use Python 3 instead", file=sys.stderr)
-
 #
 # history test is a special case
 #
@@ -43,7 +38,7 @@ def runHistoryTest(child):
     i = 0
 
     # fill the history with numbers
-    while i < history_size / 10:
+    while i < history_size // 10:
         # add 1 to prevent from parsing as octals
         child.send("1" + str(i).zfill(8) + cmdline_test_data.ENTER)
         # the app will simply print out the number
diff --git a/app/test-cmdline/cmdline_test_data.py b/app/test-cmdline/cmdline_test_data.py
index 114d2cb6a0..2d9b3262a6 100644
--- a/app/test-cmdline/cmdline_test_data.py
+++ b/app/test-cmdline/cmdline_test_data.py
@@ -1,3 +1,4 @@
+#!/usr/bin/env python3
 # SPDX-License-Identifier: BSD-3-Clause
 # Copyright(c) 2010-2014 Intel Corporation
 
diff --git a/app/test/autotest.py b/app/test/autotest.py
index cf7584ccd7..9eef1efbe5 100644
--- a/app/test/autotest.py
+++ b/app/test/autotest.py
@@ -1,9 +1,8 @@
-#!/usr/bin/env python
+#!/usr/bin/env python3
 # SPDX-License-Identifier: BSD-3-Clause
 # Copyright(c) 2010-2014 Intel Corporation
 
 # Script that uses either test app or qemu controlled by python-pexpect
-from __future__ import print_function
 import autotest_data
 import autotest_runner
 import sys
@@ -17,10 +16,6 @@ def usage():
     usage()
     sys.exit(1)
 
-if sys.version_info.major < 3:
-    print("WARNING: Python 2 is deprecated for use in DPDK, and will not work in future releases.", file=sys.stderr)
-    print("Please use Python 3 instead", file=sys.stderr)
-
 target = sys.argv[2]
 
 test_whitelist = None
diff --git a/app/test/autotest_data.py b/app/test/autotest_data.py
index 4b7da45e09..097638941f 100644
--- a/app/test/autotest_data.py
+++ b/app/test/autotest_data.py
@@ -1,3 +1,4 @@
+#!/usr/bin/env python3
 # SPDX-License-Identifier: BSD-3-Clause
 # Copyright(c) 2010-2014 Intel Corporation
 
diff --git a/app/test/autotest_runner.py b/app/test/autotest_runner.py
index 95e74c760d..998fe57a55 100644
--- a/app/test/autotest_runner.py
+++ b/app/test/autotest_runner.py
@@ -1,10 +1,10 @@
+#!/usr/bin/env python3
 # SPDX-License-Identifier: BSD-3-Clause
 # Copyright(c) 2010-2014 Intel Corporation
 
 # The main logic behind running autotests in parallel
 
-from __future__ import print_function
-import StringIO
+import io
 import csv
 from multiprocessing import Pool, Queue
 import pexpect
@@ -50,11 +50,7 @@ def first_cpu_on_node(node_nr):
                 map(os.path.basename, cpu_path)
             )
     )
-    # for compatibility between python 3 and 2 we need to make interable out
-    # of filter return as it returns list in python 2 and a generator in 3
-    m = next(iter(cpu_name))
-    return int(m.group(1))
-
+    return int(next(cpu_name).group(1))
 
 pool_child = None  # per-process child
 
@@ -78,7 +74,7 @@ def pool_init(queue, result_queue):
     cmdline = "%s %s" % (cmdline, prefix_cmdline)
 
     # prepare logging of init
-    startuplog = StringIO.StringIO()
+    startuplog = io.StringIO()
 
     # run test app
     try:
@@ -86,8 +82,7 @@ def pool_init(queue, result_queue):
         print("\n%s %s\n" % ("=" * 20, prefix), file=startuplog)
         print("\ncmdline=%s" % cmdline, file=startuplog)
 
-        pool_child = pexpect.spawn(cmdline, logfile=startuplog)
-
+        pool_child = pexpect.spawn(cmdline, logfile=startuplog, encoding='utf-8')
         # wait for target to boot
         if not wait_prompt(pool_child):
             pool_child.close()
@@ -138,7 +133,7 @@ def run_test(target, test):
     # create log buffer for each test
     # in multiprocessing environment, the logging would be
     # interleaved and will create a mess, hence the buffering
-    logfile = StringIO.StringIO()
+    logfile = io.StringIO()
     pool_child.logfile = logfile
 
     # make a note when the test started
@@ -210,9 +205,9 @@ def __init__(self, cmdline, target, blacklist, whitelist, n_processes):
         # parse the binary for available test commands
         binary = cmdline.split()[0]
         stripped = 'not stripped' not in \
-                   subprocess.check_output(['file', binary])
+                   subprocess.check_output(['file', binary]).decode()
         if not stripped:
-            symbols = subprocess.check_output(['nm', binary]).decode('utf-8')
+            symbols = subprocess.check_output(['nm', binary]).decode()
             self.avail_cmds = re.findall('test_register_(\w+)', symbols)
         else:
             self.avail_cmds = None
diff --git a/app/test/autotest_test_funcs.py b/app/test/autotest_test_funcs.py
index 26688b7132..775dfd1dc5 100644
--- a/app/test/autotest_test_funcs.py
+++ b/app/test/autotest_test_funcs.py
@@ -1,3 +1,4 @@
+#!/usr/bin/env python3
 # SPDX-License-Identifier: BSD-3-Clause
 # Copyright(c) 2010-2014 Intel Corporation
 
diff --git a/buildtools/map_to_win.py b/buildtools/map_to_win.py
index 2990b58634..2a6cb88605 100644
--- a/buildtools/map_to_win.py
+++ b/buildtools/map_to_win.py
@@ -1,8 +1,7 @@
-#!/usr/bin/env python
+#!/usr/bin/env python3
 # SPDX-License-Identifier: BSD-3-Clause
 # Copyright(c) 2019 Intel Corporation
 
-from __future__ import print_function
 import sys
 from os.path import dirname, basename, join, exists
 
diff --git a/config/arm/armv8_machine.py b/config/arm/armv8_machine.py
index 404866d2f8..1f689d9a83 100755
--- a/config/arm/armv8_machine.py
+++ b/config/arm/armv8_machine.py
@@ -1,4 +1,4 @@
-#!/usr/bin/python
+#!/usr/bin/env python3
 # SPDX-License-Identifier: BSD-3-Clause
 # Copyright(c) 2017 Cavium, Inc
 
diff --git a/devtools/update_version_map_abi.py b/devtools/update_version_map_abi.py
index 10c3bc8098..3536a54b44 100755
--- a/devtools/update_version_map_abi.py
+++ b/devtools/update_version_map_abi.py
@@ -1,4 +1,4 @@
-#!/usr/bin/env python
+#!/usr/bin/env python3
 # SPDX-License-Identifier: BSD-3-Clause
 # Copyright(c) 2019 Intel Corporation
 
@@ -9,7 +9,6 @@
 from the devtools/update-abi.sh utility.
 """
 
-from __future__ import print_function
 import argparse
 import sys
 import re
@@ -160,10 +159,6 @@ def __generate_internal_abi(f_out, lines):
     print("};", file=f_out)
 
 def __main():
-    if sys.version_info.major < 3:
-        print("WARNING: Python 2 is deprecated for use in DPDK, and will not work in future releases.", file=sys.stderr)
-        print("Please use Python 3 instead", file=sys.stderr)
-
     arg_parser = argparse.ArgumentParser(
         description='Merge versions in linker version script.')
 
diff --git a/doc/guides/conf.py b/doc/guides/conf.py
index 9ebc26ed3f..ef550f68c0 100644
--- a/doc/guides/conf.py
+++ b/doc/guides/conf.py
@@ -1,7 +1,7 @@
+#!/usr/bin/env python3
 # SPDX-License-Identifier: BSD-3-Clause
 # Copyright(c) 2010-2015 Intel Corporation
 
-from __future__ import print_function
 from docutils import nodes
 from distutils.version import LooseVersion
 from sphinx import __version__ as sphinx_version
@@ -13,12 +13,7 @@
 from os.path import dirname
 from os.path import join as path_join
 
-try:
-    # Python 2.
-    import ConfigParser as configparser
-except:
-    # Python 3.
-    import configparser
+import configparser
 
 try:
     import sphinx_rtd_theme
diff --git a/doc/guides/contributing/coding_style.rst b/doc/guides/contributing/coding_style.rst
index 0be9546a6a..d19b646fc6 100644
--- a/doc/guides/contributing/coding_style.rst
+++ b/doc/guides/contributing/coding_style.rst
@@ -765,7 +765,7 @@ specializations, run the ``app/test`` binary, and use the ``dump_log_types``
 Python Code
 -----------
 
-All Python code should work with Python 2.7+ and 3.2+ and be compliant with
+All Python code should be compliant with
 `PEP8 (Style Guide for Python Code) <https://www.python.org/dev/peps/pep-0008/>`_.
 
 The ``pep8`` tool can be used for testing compliance with the guidelines.
diff --git a/doc/guides/rel_notes/deprecation.rst b/doc/guides/rel_notes/deprecation.rst
index 29cb782178..0be208edca 100644
--- a/doc/guides/rel_notes/deprecation.rst
+++ b/doc/guides/rel_notes/deprecation.rst
@@ -233,12 +233,6 @@ Deprecation Notices
   In this case the function will return -1 unless the environment is unset first
   (using ``rte_power_unset_env``). Other function usage scenarios will not change.
 
-* python: Since the beginning of 2020, Python 2 has officially reached
-  end-of-support: https://www.python.org/doc/sunset-python-2/.
-  Python 2 support will be completely removed in 20.11.
-  In 20.08, explicit deprecation warnings will be displayed when running
-  scripts with Python 2.
-
 * dpdk-setup.sh: This old script relies on deprecated stuff, and especially
   ``make``. Given environments are too much variables for such a simple script,
   it will be removed in DPDK 20.11.
diff --git a/doc/guides/rel_notes/release_20_11.rst b/doc/guides/rel_notes/release_20_11.rst
index c2175f37f3..4e61431c6c 100644
--- a/doc/guides/rel_notes/release_20_11.rst
+++ b/doc/guides/rel_notes/release_20_11.rst
@@ -122,6 +122,8 @@ Removed Items
 
 * vhost: Dequeue zero-copy support has been removed.
 
+* Removed Python 2 support since it was EOL'd in January 2020.
+
 
 API Changes
 -----------
diff --git a/usertools/cpu_layout.py b/usertools/cpu_layout.py
index 5423c7965f..cc39638213 100755
--- a/usertools/cpu_layout.py
+++ b/usertools/cpu_layout.py
@@ -1,19 +1,8 @@
-#!/usr/bin/env python
+#!/usr/bin/env python3
 # SPDX-License-Identifier: BSD-3-Clause
 # Copyright(c) 2010-2014 Intel Corporation
 # Copyright(c) 2017 Cavium, Inc. All rights reserved.
 
-from __future__ import print_function
-import sys
-try:
-    xrange # Python 2
-except NameError:
-    xrange = range # Python 3
-
-if sys.version_info.major < 3:
-    print("WARNING: Python 2 is deprecated for use in DPDK, and will not work in future releases.", file=sys.stderr)
-    print("Please use Python 3 instead", file=sys.stderr)
-
 sockets = []
 cores = []
 core_map = {}
@@ -21,7 +10,7 @@
 fd = open("{}/kernel_max".format(base_path))
 max_cpus = int(fd.read())
 fd.close()
-for cpu in xrange(max_cpus + 1):
+for cpu in range(max_cpus + 1):
     try:
         fd = open("{}/cpu{}/topology/core_id".format(base_path, cpu))
     except IOError:
diff --git a/usertools/dpdk-devbind.py b/usertools/dpdk-devbind.py
index 094c2ffc8b..8278a748d4 100755
--- a/usertools/dpdk-devbind.py
+++ b/usertools/dpdk-devbind.py
@@ -1,9 +1,8 @@
-#! /usr/bin/env python
+#!/usr/bin/env python3
 # SPDX-License-Identifier: BSD-3-Clause
 # Copyright(c) 2010-2014 Intel Corporation
 #
 
-from __future__ import print_function
 import sys
 import os
 import getopt
@@ -12,10 +11,6 @@
 from os.path import exists, abspath, dirname, basename
 from os.path import join as path_join
 
-if sys.version_info.major < 3:
-    print("WARNING: Python 2 is deprecated for use in DPDK, and will not work in future releases.", file=sys.stderr)
-    print("Please use Python 3 instead", file=sys.stderr)
-
 # The PCI base class for all devices
 network_class = {'Class': '02', 'Vendor': None, 'Device': None,
                     'SVendor': None, 'SDevice': None}
@@ -154,14 +149,6 @@ def usage():
 
     """ % locals())  # replace items from local variables
 
-
-# This is roughly compatible with check_output function in subprocess module
-# which is only available in python 2.7.
-def check_output(args, stderr=None):
-    '''Run a command and capture its output'''
-    return subprocess.Popen(args, stdout=subprocess.PIPE,
-                            stderr=stderr).communicate()[0]
-
 # check if a specific kernel module is loaded
 def module_is_loaded(module):
     global loaded_modules
@@ -218,8 +205,7 @@ def get_pci_device_details(dev_id, probe_lspci):
     device = {}
 
     if probe_lspci:
-        extra_info = check_output(["lspci", "-vmmks", dev_id]).splitlines()
-
+        extra_info = subprocess.check_output(["lspci", "-vmmks", dev_id]).splitlines()
         # parse lspci details
         for line in extra_info:
             if len(line) == 0:
@@ -255,7 +241,7 @@ def get_device_details(devices_type):
     # first loop through and read details for all devices
     # request machine readable format, with numeric IDs and String
     dev = {}
-    dev_lines = check_output(["lspci", "-Dvmmnnk"]).splitlines()
+    dev_lines = subprocess.check_output(["lspci", "-Dvmmnnk"]).splitlines()
     for dev_line in dev_lines:
         if len(dev_line) == 0:
             if device_type_match(dev, devices_type):
@@ -283,7 +269,7 @@ def get_device_details(devices_type):
         # check what is the interface if any for an ssh connection if
         # any to this host, so we can mark it later.
         ssh_if = []
-        route = check_output(["ip", "-o", "route"])
+        route = subprocess.check_output(["ip", "-o", "route"])
         # filter out all lines for 169.254 routes
         route = "\n".join(filter(lambda ln: not ln.startswith("169.254"),
                              route.decode().splitlines()))
diff --git a/usertools/dpdk-pmdinfo.py b/usertools/dpdk-pmdinfo.py
index f9ed755176..1661982791 100755
--- a/usertools/dpdk-pmdinfo.py
+++ b/usertools/dpdk-pmdinfo.py
@@ -1,4 +1,4 @@
-#!/usr/bin/env python
+#!/usr/bin/env python3
 # SPDX-License-Identifier: BSD-3-Clause
 # Copyright(c) 2016  Neil Horman <nhorman@tuxdriver.com>
 
@@ -7,8 +7,6 @@
 # Utility to dump PMD_INFO_STRING support from an object file
 #
 # -------------------------------------------------------------------------
-from __future__ import print_function
-from __future__ import unicode_literals
 import json
 import io
 import os
@@ -28,9 +26,6 @@
 pcidb = None
 
 # ===========================================
-if sys.version_info.major < 3:
-        print("WARNING: Python 2 is deprecated for use in DPDK, and will not work in future releases.", file=sys.stderr)
-        print("Please use Python 3 instead", file=sys.stderr)
 
 class Vendor:
     """
diff --git a/usertools/dpdk-telemetry-client.py b/usertools/dpdk-telemetry-client.py
index 98d28fa89b..d8e439027c 100755
--- a/usertools/dpdk-telemetry-client.py
+++ b/usertools/dpdk-telemetry-client.py
@@ -1,10 +1,7 @@
-#! /usr/bin/env python
+#! /usr/bin/env python3
 # SPDX-License-Identifier: BSD-3-Clause
 # Copyright(c) 2018 Intel Corporation
 
-from __future__ import print_function
-from __future__ import unicode_literals
-
 import socket
 import os
 import sys
@@ -18,15 +15,6 @@
 GLOBAL_METRICS_REQ = "{\"action\":0,\"command\":\"global_stat_values\",\"data\":null}"
 DEFAULT_FP = "/var/run/dpdk/default_client"
 
-try:
-    raw_input  # Python 2
-except NameError:
-    raw_input = input  # Python 3
-
-if sys.version_info.major < 3:
-    print("WARNING: Python 2 is deprecated for use in DPDK, and will not work in future releases.", file=sys.stderr)
-    print("Please use Python 3 instead", file=sys.stderr)
-
 class Socket:
 
     def __init__(self):
@@ -86,7 +74,7 @@ def requestMetrics(self): # Requests metrics for given client
 
     def repeatedlyRequestMetrics(self, sleep_time): # Recursively requests metrics for given client
         print("\nPlease enter the number of times you'd like to continuously request Metrics:")
-        n_requests = int(raw_input("\n:"))
+        n_requests = int(input("\n:"))
         print("\033[F") #Removes the user input from screen, cleans it up
         print("\033[K")
         for i in range(n_requests):
@@ -107,7 +95,7 @@ def interactiveMenu(self, sleep_time): # Creates Interactive menu within the scr
             print("[4] Unregister client")
 
             try:
-                self.choice = int(raw_input("\n:"))
+                self.choice = int(input("\n:"))
                 print("\033[F") #Removes the user input for screen, cleans it up
                 print("\033[K")
                 if self.choice == 1:
diff --git a/usertools/dpdk-telemetry.py b/usertools/dpdk-telemetry.py
index 8e4039d574..181859658f 100755
--- a/usertools/dpdk-telemetry.py
+++ b/usertools/dpdk-telemetry.py
@@ -1,4 +1,4 @@
-#! /usr/bin/python3
+#! /usr/bin/env python3
 # SPDX-License-Identifier: BSD-3-Clause
 # Copyright(c) 2020 Intel Corporation